TY - JOUR A1 - Rodriguez Rigueiro, Teresa AU - Valladares Ayerbes, Manuel AU - Haz Conde, María del Mar AU - Blanco Calvo, Moisés AU - Aparicio Gallego, Guadalupe AU - Fernández Puente, Patricia AU - BLANCO GARCIA, FRANCISCO JAVIER AU - Aparicio Gallego, Guadalupe AU - Lorenzo Lorenzo, María Jesus AU - Antón Aparicio, Luis Miguel AU - Figueroa, Angélica T1 - A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues Y1 - 2011 SN - 1615-9853 UR - http://hdl.handle.net/20.500.11940/3116 AB - Most of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry in a clinical routine as it is difficult to recover intact protein from these FFPE tissues. Here, we report a novel, short time-consuming and cost-effective method to extract full-length, non-degraded proteins from FFPE tissues. This procedure is combined with an effective and non-toxic deparaffinisation process and an extraction method based on antigen-retrieval, high concentration of SDS and high temperature. We have obtained enough intact protein to be detected by Western blotting analysis. This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases. LA - eng ER -