TY - JOUR 
A1 - Rodriguez Rigueiro, Teresa
AU - Valladares Ayerbes, Manuel
AU - Haz Conde, María del Mar
AU - Blanco Calvo, Moisés
AU - Aparicio Gallego, Guadalupe
AU - Fernández Puente, Patricia
AU - BLANCO GARCIA, FRANCISCO JAVIER
AU - Aparicio Gallego, Guadalupe
AU - Lorenzo Lorenzo, María Jesus
AU - Antón Aparicio, Luis Miguel
AU - Figueroa, Angélica
T1 - A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues
Y1 - 2011
SN - 1615-9853
UR - http://hdl.handle.net/20.500.11940/3116
AB - Most of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry in a clinical routine as it is difficult to recover intact protein from these FFPE tissues. Here, we report a novel, short time-consuming and cost-effective method to extract full-length, non-degraded proteins from FFPE tissues. This procedure is combined with an effective and non-toxic deparaffinisation process and an extraction method based on antigen-retrieval, high concentration of SDS and high temperature. We have obtained enough intact protein to be detected by Western blotting analysis. This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases.
LA - eng
ER -