TY - JOUR A1 - Roca Rivada, Arturo AU - Bravo López, Susana Belén AU - Pérez Sotelo, Diego AU - Alonso Lorenzo, Jana AU - Castro Pais, Ana Isabel AU - Baamonde de la Torre, Ivan AU - Baltar Boileve, Javier AU - Casanueva Freijo, Felipe AU - Pardo Pumar, María Isabel T1 - CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators Y1 - 2015 SN - 2045-2322 UR - http://hdl.handle.net/20.500.11940/8341 AB - In the context of obesity, strong evidences support a distinctive pathological contribution of adipose tissue depending on its anatomical site of accumulation. Therefore, subcutaneous adipose tissue (SAT) has been lately considered metabolically benign compared to visceral fat (VAT), whose location is associated to the risk of developing cardiovascular disease, insulin resistance, and other associated comorbidities. Under the above situation, the chronic local inflammation that characterizes obese adipose tissue, has acquired a major role on the pathogenesis of obesity. In this work, we have analyzed for the first time human obese VAT and SAT secretomes using an improved quantitative proteomic approach for the study of tissue secretomes, Comparison of Isotope-Labeled Amino acid Incorporation Rates (CILAIR). The use of double isotope-labeling-CILAIR approach to analyze VAT and SAT secretomes allowed the identification of location-specific secreted proteins and its differential secretion. Additionally to the very high percentage of identified proteins previously implicated in obesity or in its comorbidities, this approach was revealed as a useful tool for the study of the obese adipose tissue microenvironment including extracellular matrix (ECM) remodeling and inflammatory status. The results herein presented reinforce the fact that VAT and SAT depots have distinct features and contribute differentially to metabolic disease. KW - Adipose Tissue KW - Amino Acids KW - Extracellular Matrix KW - Humans KW - Inflammation KW - Inflammation Mediators KW - Insulin Resistance KW - Intra-Abdominal Fat KW - Isotope Labeling KW - Obesity KW - Proteomics KW - Subcutaneous Fat LA - eng ER -