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dc.contributor.authorGómez Carballa, Alberto
dc.contributor.authorCebey López, Miriam
dc.contributor.authorPardo Seco, Jacobo José
dc.contributor.authorBarral Arca, Ruth
dc.contributor.authorRIVERO CALLE, IRENE 
dc.contributor.authorPischedda ., Sara
dc.contributor.authorCurrás Tuala, María José
dc.contributor.authorGOMEZ RIAL, JOSE 
dc.contributor.authorBarros Angueira, Francisco
dc.contributor.authorMartinón Torres, Federico 
dc.contributor.authorSalas Ellacuriaga, Antonio
dc.date.accessioned2021-10-07T08:56:37Z
dc.date.available2021-10-07T08:56:37Z
dc.date.issued2019
dc.identifier.issn2045-2322
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/31409879es
dc.identifier.urihttp://hdl.handle.net/20.500.11940/15470
dc.description.abstractThe diagnosis of bacterial infections in hospital settings is currently performed using bacterial culture from sterile site, but they are lengthy and limited. Transcriptomic biomarkers are becoming promising tools for diagnosis with potential applicability in clinical settings. We evaluated a RT-qPCR assay for a 2-transcript host expression signature (FAM89A and IFI44L genes) inferred from microarray data that allow to differentiate between viral and bacterial infection in febrile children. This assay was able to discriminate viral from bacterial infections (P-value = 1.04 x 10(-4); AUC = 92.2%; sensitivity = 90.9%; specificity = 85.7%) and showed very high reproducibility regardless of the reference gene(s) used to normalize the data. Unexpectedly, the monogenic IFI44L expression signature yielded better results than those obtained from the 2-transcript test (P-value = 3.59 x 10(-5); AUC = 94.1%; sensitivity = 90.9%; specificity = 92.8%). We validated this IFI44L signature in previously published microarray and whole-transcriptome data from patients affected by different types of viral and bacterial infections, confirming that this gene alone differentiates between both groups, thus saving time, effort, and costs. Herein, we demonstrate that host expression microarray data can be successfully translated into a fast, highly accurate and relatively inexpensive in vitro assay that could be implemented in the clinical routine.en
dc.language.isoeng
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleA qPCR expression assay of IFI44L gene differentiates viral from bacterial infections in febrile childrenes
dc.typeArtigoes
dc.authorsophosGómez-Carballa, A.
dc.authorsophosCebey-López, M.
dc.authorsophosPardo-Seco, J.
dc.authorsophosBarral-Arca, R.
dc.authorsophosRivero-Calle, I.
dc.authorsophosPischedda, S.
dc.authorsophosCurrás-Tuala, M. J.
dc.authorsophosGómez-Rial, J.
dc.authorsophosBarros, F.
dc.authorsophosMartinón-Torres, F.
dc.authorsophosSalas, A.
dc.identifier.doi10.1038/s41598-019-48162-9
dc.identifier.pmid31409879
dc.identifier.sophos30780
dc.issue.number1es
dc.journal.titleScientific Reportses
dc.organizationServizo Galego de Saúde::Dirección Xeral de Asistencia Sanitaria::Fundación Pública Galega de Medicina Xenómica
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Pediatría
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS)
dc.page.initial11780es
dc.relation.publisherversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692396/pdf/41598_2019_Article_48162.pdf
dc.rights.accessRightsopenAccess
dc.subject.keywordFPGMX
dc.subject.keywordCHUS
dc.subject.keywordIDIS
dc.typefidesArtículo Científico (incluye Original, Original breve, Revisión Sistemática y Meta-análisis)
dc.typesophosArtículo Original
dc.volume.number9es


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