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A qPCR expression assay of IFI44L gene differentiates viral from bacterial infections in febrile children
dc.contributor.author | Gómez Carballa, Alberto | |
dc.contributor.author | Cebey López, Miriam | |
dc.contributor.author | Pardo Seco, Jacobo José | |
dc.contributor.author | Barral Arca, Ruth | |
dc.contributor.author | RIVERO CALLE, IRENE | |
dc.contributor.author | Pischedda ., Sara | |
dc.contributor.author | Currás Tuala, María José | |
dc.contributor.author | GOMEZ RIAL, JOSE | |
dc.contributor.author | Barros Angueira, Francisco | |
dc.contributor.author | Martinón Torres, Federico | |
dc.contributor.author | Salas Ellacuriaga, Antonio | |
dc.date.accessioned | 2021-10-07T08:56:37Z | |
dc.date.available | 2021-10-07T08:56:37Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | https://www.ncbi.nlm.nih.gov/pubmed/31409879 | es |
dc.identifier.uri | http://hdl.handle.net/20.500.11940/15470 | |
dc.description.abstract | The diagnosis of bacterial infections in hospital settings is currently performed using bacterial culture from sterile site, but they are lengthy and limited. Transcriptomic biomarkers are becoming promising tools for diagnosis with potential applicability in clinical settings. We evaluated a RT-qPCR assay for a 2-transcript host expression signature (FAM89A and IFI44L genes) inferred from microarray data that allow to differentiate between viral and bacterial infection in febrile children. This assay was able to discriminate viral from bacterial infections (P-value = 1.04 x 10(-4); AUC = 92.2%; sensitivity = 90.9%; specificity = 85.7%) and showed very high reproducibility regardless of the reference gene(s) used to normalize the data. Unexpectedly, the monogenic IFI44L expression signature yielded better results than those obtained from the 2-transcript test (P-value = 3.59 x 10(-5); AUC = 94.1%; sensitivity = 90.9%; specificity = 92.8%). We validated this IFI44L signature in previously published microarray and whole-transcriptome data from patients affected by different types of viral and bacterial infections, confirming that this gene alone differentiates between both groups, thus saving time, effort, and costs. Herein, we demonstrate that host expression microarray data can be successfully translated into a fast, highly accurate and relatively inexpensive in vitro assay that could be implemented in the clinical routine. | en |
dc.language.iso | eng | |
dc.rights | Atribución 4.0 Internacional | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | A qPCR expression assay of IFI44L gene differentiates viral from bacterial infections in febrile children | es |
dc.type | Artigo | es |
dc.authorsophos | Gómez-Carballa, A. | |
dc.authorsophos | Cebey-López, M. | |
dc.authorsophos | Pardo-Seco, J. | |
dc.authorsophos | Barral-Arca, R. | |
dc.authorsophos | Rivero-Calle, I. | |
dc.authorsophos | Pischedda, S. | |
dc.authorsophos | Currás-Tuala, M. J. | |
dc.authorsophos | Gómez-Rial, J. | |
dc.authorsophos | Barros, F. | |
dc.authorsophos | Martinón-Torres, F. | |
dc.authorsophos | Salas, A. | |
dc.identifier.doi | 10.1038/s41598-019-48162-9 | |
dc.identifier.pmid | 31409879 | |
dc.identifier.sophos | 30780 | |
dc.issue.number | 1 | es |
dc.journal.title | Scientific Reports | es |
dc.organization | Servizo Galego de Saúde::Dirección Xeral de Asistencia Sanitaria::Fundación Pública Galega de Medicina Xenómica | |
dc.organization | Servizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Pediatría | |
dc.organization | Servizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS) | |
dc.page.initial | 11780 | es |
dc.relation.publisherversion | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6692396/pdf/41598_2019_Article_48162.pdf | |
dc.rights.accessRights | openAccess | |
dc.subject.keyword | FPGMX | |
dc.subject.keyword | CHUS | |
dc.subject.keyword | IDIS | |
dc.typefides | Artículo Científico (incluye Original, Original breve, Revisión Sistemática y Meta-análisis) | |
dc.typesophos | Artículo Original | |
dc.volume.number | 9 | es |