Show simple item record

dc.contributor.authorRapado González, Oscar
dc.contributor.authorMajem, B.
dc.contributor.authorALVAREZ CASTRO, ANA MARIA 
dc.contributor.authorDíaz Peña, Roberto
dc.contributor.authorAbalo Piñeiro, Alicia
dc.contributor.authorSuárez-Cabrera, L.
dc.contributor.authorSantamaría, A.
dc.contributor.authorLópez López, Rafael 
dc.contributor.authorMuinelo Romay , Laura
dc.contributor.authorSuárez Cunqueiro, María Mercedes
dc.date.accessioned2021-10-14T09:19:14Z
dc.date.available2021-10-14T09:19:14Z
dc.date.issued2019
dc.identifier.issn2077-0383
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/31757017
dc.identifier.urihttp://hdl.handle.net/20.500.11940/15535
dc.description.abstractSalivary microRNAs (miRNAs) are of high interest as diagnostic biomarkers for non-oral cancer. However, little is known about their value for colorectal cancer (CRC) detection. Our study aims to characterize salivary miRNAs in order to identify non-invasive markers for CRC diagnosis. The screening of 754 miRNAs was performed in saliva samples from 14 CRC and 10 healthy controls. The differential expressed miRNAs were validated by RT-qPCR in 51 CRC, 19 adenomas and 37 healthy controls. Receiver operating characteristic (ROC) curves and logistic regression models were performed to analyze the clinical value of these miRNAs. Twenty-two salivary miRNAs were significantly deregulated in CRC patients vs. healthy individuals (P < 0.05) in the discovery phase. From those, five upregulated miRNAs (miR-186-5p, miR-29a-3p, miR-29c-3p, miR-766-3p, and miR-491-5p) were confirmed to be significantly higher in the CRC vs. healthy group (P < 0.05). This five-miRNA signature showed diagnostic value (72% sensitivity, 66.67% specificity, AUC = 0.754) to detect CRC, which was even higher in combination with carcinoembryonic antigen (CEA) levels. Overall, after the first global characterization of salivary miRNAs in CRC, a five-miRNA panel was identified as a promising tool to diagnose this malignancy, representing a novel approach to detect cancer-associated epigenetic alterations using a non-invasive strategy.
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleA Novel Saliva-Based miRNA Signature for Colorectal Cancer Diagnosis
dc.typeArtigoes
dc.authorsophosRapado González, Oscar
dc.authorsophosMajem, B.
dc.authorsophosAlvarez Castro, Ana Maria
dc.authorsophosDíaz Peña, Roberto
dc.authorsophosAbalo Piñeiro, Alicia
dc.authorsophosSuárez-Cabrera, L.
dc.authorsophosSantamaría, A.
dc.authorsophosLópez López, Rafael
dc.authorsophosMuinelo Romay , Laura
dc.authorsophosSuárez Cunqueiro, María Mercedes
dc.identifier.doi10.3390/jcm8122029
dc.identifier.pmid31757017
dc.identifier.sophos30936
dc.issue.number12
dc.journal.titleJournal of Clinical Medicine
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Dixestivo
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Oncoloxía médica
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS)
dc.relation.publisherversionhttps://res.mdpi.com/d_attachment/jcm/jcm-08-02029/article_deploy/jcm-08-02029-v2.pdf
dc.rights.accessRightsopenAccess
dc.subject.keywordCHUS
dc.subject.keywordIDIS
dc.typefidesArtículo Científico (incluye Original, Original breve, Revisión Sistemática y Meta-análisis)
dc.typesophosArtículo Original
dc.volume.number8


Files in this item

This item appears in the following Collection(s)

Show simple item record

Atribución 4.0 Internacional
Except where otherwise noted, this item's license is described as Atribución 4.0 Internacional