FNDC5/Irisin counteracts lipotoxic-induced apoptosis in hypoxic H9c2 cells
Identificadores
Identificadores
URI: http://hdl.handle.net/20.500.11940/15697
PMID: 31284265
DOI: 10.1530/jme-19-0123
ISSN: 0952-5041
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Visualización o descarga de ficheros
Fecha de publicación
2019Título de revista
JOURNAL OF MOLECULAR ENDOCRINOLOGY
Tipo de contenido
Artigo
DeCS
infarto de miocardio | apoptosis | animales | fibronectinas | supervivencia celular | ácidos grasos | línea celular | estrés oxidativo | transducción de señales | ratas | proteínas protooncogénicas c-aktMeSH
Apoptosis | Rats | Cell Line | Proto-Oncogene Proteins c-akt | Signal Transduction | Animals | Fibronectins | Fatty Acids | Cell Survival | Oxidative Stress | Myocardial InfarctionResumen
Irisin is a newly identified adipokine critical to modulate body metabolism, fatty acid metabolism and oxidative stress; recent evidence suggests a cardioprotective role in ischemic injury. Loss of cardiomyocytes during acute myocardial infarction is strongly associated with energetic changes and lipotoxic-induced apoptosis. Our aim was to study FNDC5/irisin's potential protective role against hypoxia and lipotoxicity, both related with myocardial infarction environment. H9c2 cells were treated with palmitate and/or irisin in normoxic/hypoxic conditions. Cell viability and apoptosis were assessed by MTT assay and annexin V/PI staining. Immunoblotting was used to confirm apoptotic cascade regulation. Irisin counteracts lipotoxic-induced apoptosis in hypoxic cardiomyoblasts by activating Akt signaling pathway suggesting the potential therapeutic role of irisin in ischemic heart disease.