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dc.contributor.authorFernández Puente, Patricia 
dc.contributor.authorGonzález Rodríguez, Lucia
dc.contributor.authorCalamia ., Valentina
dc.contributor.authorPicchi Figueira, Florencia Cristina
dc.contributor.authorLourido Salas, Lucía
dc.contributor.authorCamacho Encina, María
dc.contributor.authorOREIRO VILLAR, NATIVIDAD 
dc.contributor.authorRocha Loureda, Beatriz
dc.contributor.authorPaz González, Rocio
dc.contributor.authorMarina, Anabel
dc.contributor.authorGarcia, Carlos
dc.contributor.authorBLANCO GARCIA, FRANCISCO JAVIER 
dc.contributor.authorRuiz Romero, Cristina 
dc.date.accessioned2022-01-25T12:16:41Z
dc.date.available2022-01-25T12:16:41Z
dc.date.issued2019
dc.identifier.issn1535-9476
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/31352363es
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773562/pdf/zjw2018.pdfes
dc.identifier.urihttp://hdl.handle.net/20.500.11940/15911
dc.description.abstractOsteoarthritis (OA) is a pathology characterized by the loss of articular cartilage. In this study, we performed a peptidomic strategy to identify endogenous peptides (neopeptides) that are released from human osteoarthritic tissue, which may serve as disease markers. With this aim, secretomes of osteoarthritic and healthy articular cartilages obtained from knee and hip were analyzed by shotgun peptidomics. This discovery step led to the identification of 1175 different peptides, corresponding to 101 proteins, as products of the physiological or pathological turnover of cartilage extracellular matrix. Then, a targeted multiple reaction monitoring-mass spectrometry method was developed to quantify the panel of best marker candidates on a larger set of samples (n = 62). Statistical analyses were performed to evaluate the significance of the observed differences and the ability of the neopeptides to classify the tissue. Eight of them were differentially abundant in the media from wounded zones of OA cartilage compared with the healthy tissue (p < 0.05). Three neopeptides belonging to Clusterin and one from Cartilage Oligomeric Matrix Protein showed a disease-dependent decrease specifically in hip OA, whereas two from Prolargin (PRELP) and one from Cartilage Intermediate Layer Protein 1 were significantly increased in samples from knee OA. The release of one peptide from PRELP showed the best metrics for tissue classification (AUC = 0.834). The present study reveals specific neopeptides that are differentially released from knee or hip human osteoarthritic cartilage compared with healthy tissue. This evidences the intervention of characteristic pathogenic pathways in OA and provides a novel panel of peptidic candidates for biomarker development.en
dc.language.isoenges
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshHumans *
dc.subject.meshTandem Mass Spectrometry *
dc.subject.meshPeptides *
dc.subject.meshCells *
dc.subject.meshExtracellular Matrix *
dc.subject.meshCulture Media *
dc.subject.meshCase-Control Studies *
dc.subject.meshProteomics *
dc.subject.meshOsteoarthritis *
dc.subject.meshAged *
dc.subject.meshOrgan Specificity *
dc.subject.meshChromatography *
dc.subject.meshCartilage *
dc.titleAnalysis of Endogenous Peptides Released from Osteoarthritic Cartilage Unravels Novel Pathogenic Markersen
dc.typeArtigoes
dc.identifier.doi10.1074/mcp.RA119.001554
dc.identifier.pmid31352363
dc.identifier.sophos32335
dc.issue.number10es
dc.journal.titleMOLECULAR & CELLULAR PROTEOMICSes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Biomédica da Coruña (INIBIC) Reumatoloxíaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Biomédica da Coruña (INIBIC)es
dc.rights.accessRightsopenAccesses
dc.subject.decsestudios de casos y controles *
dc.subject.decsanciano *
dc.subject.decsmedios de cultivo *
dc.subject.decscartílago *
dc.subject.decscromatografía *
dc.subject.decsmatriz extracelular *
dc.subject.decshumanos *
dc.subject.decspéptidos *
dc.subject.decscélulas *
dc.subject.decsproteómica *
dc.subject.decsespectrometría de masas en tándem *
dc.subject.decsosteoartritis *
dc.subject.decsespecificidad de órganos *
dc.subject.keywordCHUACes
dc.subject.keywordINIBICes
dc.typefidesArtículo Originales
dc.typesophosArtículo Originales
dc.volume.number18es


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Atribución 4.0 Internacional
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