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dc.contributor.authorEscalante Perez, Maria
dc.contributor.authorHermida Romero, Teresa 
dc.contributor.authorOtero Alen, Begoña
dc.contributor.authorÁlvarez Martínez, Mónica 
dc.contributor.authorFernández Prado, Ricardo 
dc.contributor.authorde la Torre Bravos, Mercedes 
dc.contributor.authorConcha Lopez, Angel 
dc.date.accessioned2022-01-25T12:17:30Z
dc.date.available2022-01-25T12:17:30Z
dc.date.issued2019
dc.identifier.issn1479-5876
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647159/pdf/12967_2019_Article_1974.pdfes
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/31331335es
dc.identifier.urihttp://hdl.handle.net/20.500.11940/15923
dc.description.abstractBACKGROUND: The use of one-step nucleic acid amplification (OSNA) allows for lymph node (LN) metastasis to be detected rapidly and accurately. We conducted a prospective single-centre clinical trial to evaluate OSNA assay in detecting LN metastasis of lung cancer. PATIENTS AND METHODS: A total of 705 LNs from 160 patients with clinical stage IA to IVA lung cancer were included in this study. The LNs were divided and submitted to routine histological diagnosis and OSNA assay and the results were compared. We also examined keratin 19 expression of different histological types lung primary tumours. RESULTS: When the cut-off value was set to 250 copies/microl, the concordance rate between the two methods was 96.17% and the sensitivity 97.14%. Discordant results were observed in 27 LNs of 21 patients. Most of these discordant results were molecular micrometastasis expressing a very low number of copies with negative histology. Most thoracic tumours were positive for keratin 19. CONCLUSIONS: Our data show that the OSNA assay might be a useful and sensitive method to diagnose LN metastasis in lung cancer and could be applied to intraoperative decision-making in personalised lung cancer surgery based on LN status and a more accurate staging of patients.en
dc.language.isoenges
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshLung Neoplasms *
dc.subject.meshAdult *
dc.subject.meshLymphatic Metastasis *
dc.subject.meshMiddle Aged *
dc.subject.meshHumans *
dc.subject.meshYoung Adult *
dc.subject.meshNucleic Acid Amplification Techniques *
dc.subject.meshKeratin-19 *
dc.subject.meshProspective Studies *
dc.subject.meshLymph Nodes *
dc.subject.meshGene Expression Regulation *
dc.subject.meshAged *
dc.titleDetection of lymph node metastasis in lung cancer patients using a one-step nucleic acid amplification assay: a single-centre prospective studyen
dc.typeArtigoes
dc.identifier.doi10.1186/s12967-019-1974-4
dc.identifier.pmid31331335
dc.identifier.sophos32384
dc.issue.number1es
dc.journal.titleJournal of Translational Medicinees
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario de A Coruña:: Anatomía Patolóxicaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario de A Coruña:: Cirurxía Torácicaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Biomédica da Coruña (INIBIC)es
dc.rights.accessRightsopenAccesses
dc.subject.decsqueratina-19 *
dc.subject.decsanciano *
dc.subject.decstécnicas de amplificación de ácidos nucleicos *
dc.subject.decsganglios linfáticos *
dc.subject.decsestudios prospectivos *
dc.subject.decsadulto joven *
dc.subject.decsmediana edad *
dc.subject.decsmetástasis linfática *
dc.subject.decshumanos *
dc.subject.decsneoplasias pulmonares *
dc.subject.decsregulación de la expresión génica *
dc.subject.decsadulto *
dc.subject.keywordCHUACes
dc.subject.keywordINIBICes
dc.typefidesArtículo Originales
dc.typesophosArtículo Originales
dc.volume.number17es


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Atribución 4.0 Internacional
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