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dc.contributor.authorRumbo Feal, Soraya
dc.contributor.authorGómez, Manuel J.
dc.contributor.authorGayoso Babio, Carmen María
dc.contributor.authorÁlvarez Fraga, Laura
dc.contributor.authorCabral, María P.
dc.contributor.authorAransay, Ana M.
dc.contributor.authorRodríguez-Ezpeleta, Naiara
dc.contributor.authorFullaondo, Ane
dc.contributor.authorValle, Jaione
dc.contributor.authorTomás Carmona, María del Mar 
dc.contributor.authorBou Arévalo, Germán 
dc.contributor.authorPoza Dominguez, Margarita
dc.date.accessioned2017-06-07T07:00:32Z
dc.date.available2017-06-07T07:00:32Z
dc.date.issued2013
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/20.500.11940/1603
dc.description.abstractAcinetobacterbaumannii has emerged as a dangerous opportunistic pathogen, with many strains able to form biofilms and thus cause persistent infections. The aim of the present study was to use high-throughput sequencing techniques to establish complete transcriptome profiles of planktonic (free-living) and sessile (biofilm) forms of A. baumannii ATCC 17978 and thereby identify differences in their gene expression patterns. Collections of mRNA from planktonic (both exponential and stationary phase cultures) and sessile (biofilm) cells were sequenced. Six mRNA libraries were prepared following the mRNA-Seq protocols from Illumina. Reads were obtained in a HiScanSQ platform and mapped against the complete genome to describe the complete mRNA transcriptomes of planktonic and sessile cells. The results showed that the gene expression pattern of A. baumannii biofilm cells was distinct from that of planktonic cells, including 1621 genes over-expressed in biofilms relative to stationary phase cells and 55 genes expressed only in biofilms. These differences suggested important changes in amino acid and fatty acid metabolism, motility, active transport, DNA-methylation, iron acquisition, transcriptional regulation, and quorum sensing, among other processes. Disruption or deletion of five of these genes caused a significant decrease in biofilm formation ability in the corresponding mutant strains. Among the genes over-expressed in biofilm cells were those in an operon involved in quorum sensing. One of them, encoding an acyl carrier protein, was shown to be involved in biofilm formation as demonstrated by the significant decrease in biofilm formation by the corresponding knockout strain. The present work serves as a basis for future studies examining the complex network systems that regulate bacterial biofilm formation and maintenance.
dc.language.isoeng
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshAcinetobacter baumannii
dc.subject.meshBiofilms
dc.subject.meshGene Expression Profiling
dc.subject.meshGene Expression Regulation, Bacterial
dc.subject.meshSequence Analysis, RNA
dc.subject.meshPlankton
dc.titleWhole transcriptome analysis of Acinetobacter baumannii assessed by RNA-sequencing reveals different mRNA expression profiles in biofilm compared to planktonic cells
dc.typeArtigoes
dc.authorsophosRumbo-Feal, S.
dc.authorsophosGomez, M. J.
dc.authorsophosGayoso, C.
dc.authorsophosAlvarez-Fraga, L.
dc.authorsophosCabral, M. P.
dc.authorsophosAransay, A. M.
dc.authorsophosRodriguez-Ezpeleta, N.
dc.authorsophosFullaondo, A.
dc.authorsophosValle, J.
dc.authorsophosTomas, M.
dc.authorsophosBou, G.
dc.authorsophosPoza, M.
dc.identifier.doi10.1371/journal.pone.0072968
dc.identifier.isi323880200056
dc.identifier.pmid24023660
dc.identifier.sophos11801
dc.issue.number8
dc.journal.titlePLoS One
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario A Coruña::Microbioloxía
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña::INIBIC.- Instituto de Investigación Biomédica
dc.page.initiale72968
dc.rights.accessRightsopenAccess
dc.subject.decsAcinetobacter baumannii
dc.subject.decsBiopelículas
dc.subject.decsPerfilación de la Expresión Génica
dc.subject.decsRegulación Bacteriana de la Expresión Génica
dc.subject.decsAnálisis de Secuencia de ARN
dc.subject.decsPlancton
dc.typesophosArtículo Original
dc.volume.number8


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