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dc.contributor.authorRodríguez Pereira, Cristina
dc.contributor.authorLagunas, Anna
dc.contributor.authorCasanellas, Ignasi
dc.contributor.authorVida, Yolanda
dc.contributor.authorPérez-Inestrosa, Ezequiel
dc.contributor.authorAndrades, José A
dc.contributor.authorBecerra, José
dc.contributor.authorSamitier, Josep
dc.contributor.authorBLANCO GARCIA, FRANCISCO JAVIER 
dc.contributor.authorMagalhaes Silva, Joana Cristina
dc.date.accessioned2022-03-08T08:51:00Z
dc.date.available2022-03-08T08:51:00Z
dc.date.issued2020
dc.identifier.issn1996-1944
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/32414175es
dc.identifier.urihttp://hdl.handle.net/20.500.11940/16218
dc.description.abstractAiming to address a stable chondrogenesis derived from mesenchymal stromal cells (MSCs) to be applied in cartilage repair strategies at the onset of osteoarthritis (OA), we analyzed the effect of arginine-glycine-aspartate (RGD) density on cell condensation that occurs during the initial phase of chondrogenesis. For this, we seeded MSC-derived from OA and healthy (H) donors in RGD-dendrimer-poly(L-lactic) acid (PLLA) nanopatterned substrates (RGD concentrations of 4 x 10(-9), 10(-8), 2.5 x 10(-8), and 10(-2) w/w), during three days and compared to a cell pellet conventional three-dimensional culture system. Molecular gene expression (collagens type-I and II-COL1A1 and COL2A1, tenascin-TNC, sex determining region Y-box9-SOX9, and gap junction protein alpha 1-GJA1) was determined as well as the cell aggregates and pellet size, collagen type-II and connexin 43 proteins synthesis. This study showed that RGD-tailored first generation dendrimer (RGD-Cys-D1) PLLA nanopatterned substrates supported the formation of pre-chondrogenic condensates from OA- and H-derived human bone marrow-MSCs with enhanced chondrogenesis regarding the cell pellet conventional system (presence of collagen type-II and connexin 43, both at the gene and protein level). A RGD-density dependent trend was observed for aggregates size, in concordance with previous studies. Moreover, the nanopatterns' had a higher effect on OA-derived MSC morphology, leading to the formation of bigger and more compact aggregates with improved expression of early chondrogenic markers.en
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleRGD-Dendrimer-Poly(L-lactic) Acid Nanopatterned Substrates for the Early Chondrogenesis of Human Mesenchymal Stromal Cells Derived from Osteoarthritic and Healthy Donorsen
dc.typeJournal Articlees
dc.authorsophosRodríguez-Pereira, Cristina;Lagunas, Anna;Casanellas, Ignasi;Vida, Yolanda;Pérez-Inestrosa, Ezequiel;Andrades, José A;Becerra, José;Samitier, Josep;Blanco, Francisco J;Magalhães, Joana
dc.identifier.doi10.3390/ma13102247
dc.identifier.pmid32414175
dc.identifier.sophos35918
dc.issue.number10es
dc.journal.titleMaterialses
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario de A Coruña::Reumatoloxíaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Biomédica da Coruña (INIBIC)es
dc.relation.publisherversionhttps://mdpi-res.com/d://attachment/materials/materials-13-02247/article://deploy/materials-13-02247-v2.pdfes
dc.rights.accessRightsopenAccess
dc.subject.keywordCHUACes
dc.subject.keywordINIBICes
dc.typefidesArtículo Originales
dc.typesophosArtículo Originales
dc.volume.number13es


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Atribución 4.0 Internacional
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