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dc.contributor.authorMayer, Celia
dc.contributor.authorMuras, Andrea
dc.contributor.authorParga, Ana
dc.contributor.authorRomero, Manuel
dc.contributor.authorRumbo Feal, Soraya
dc.contributor.authorPoza Dominguez, Margarita
dc.contributor.authorRamos-Vivas, José
dc.contributor.authorOtero, Ana
dc.date.accessioned2022-03-16T08:38:28Z
dc.date.available2022-03-16T08:38:28Z
dc.date.issued2020
dc.identifier.issn1664-302X
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/33101239es
dc.identifier.urihttp://hdl.handle.net/20.500.11940/16271
dc.description.abstractThe important nosocomial pathogen Acinetobacter baumannii presents a quorum sensing (QS) system (abaI/abaR) mediated by acyl-homoserine-lactones (AHLs) and several quorum quenching (QQ) enzymes. However, the roles of this complex network in the control of the expression of important virulence-related phenotypes such as surface-associated motility and biofilm formation is not clear. Therefore, the effect of the mutation of the AHL synthase AbaI, and the exogenous addition of the QQ enzyme Aii20J on surface-associated motility and biofilm formation by A. baumannii ATCC((R)) 17978(TM) was studied in detail. The effect of the enzyme on biofilm formation by several multidrug-resistant A. baumannii clinical isolates differing in their motility pattern was also tested. We provide evidence that a functional QS system is required for surface-associated motility and robust biofilm formation in A. baumannii ATCC((R)) 17978(TM). Important differences were found with the well-studied strain A. nosocomialis M2 regarding the relevance of the QS system depending on environmental conditions The in vitro biofilm-formation capacity of A. baumannii clinical strains was highly variable and was not related to the antibiotic resistance or surface-associated motility profiles. A high variability was also found in the sensitivity of the clinical strains to the action of the QQ enzyme, revealing important differences in virulence regulation between A. baumannii isolates and confirming that studies restricted to a single strain are not representative for the development of novel antimicrobial strategies. Extracellular DNA emerges as a key component of the extracellular matrix in A. baumannii biofilms since the combined action of the QQ enzyme Aii20J and DNase reduced biofilm formation in all tested strains. Results demonstrate that QQ strategies in combination with other enzymatic treatments such as DNase could represent an alternative approach for the prevention of A. baumannii colonization and survival on surfaces and the prevention and treatment of infections caused by this pathogen.en
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleQuorum Sensing as a Target for Controlling Surface Associated Motility and Biofilm Formation in Acinetobacter baumannii ATCC(®) 17978(TM)en
dc.typeJournal Articlees
dc.authorsophosMayer, Celia;Muras, Andrea;Parga, Ana;Romero, Manuel;Rumbo-Feal, Soraya;Poza, Margarita;Ramos-Vivas, José;Otero, Ana
dc.identifier.doi10.3389/fmicb.2020.565548
dc.identifier.pmid33101239
dc.identifier.sophos36144
dc.journal.titleFrontiers in Microbiologyes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario de A Coruña::Microbioloxíaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Biomédica da Coruña (INIBIC)es
dc.relation.publisherversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554515/pdf/fmicb-11-565548.pdfes
dc.rights.accessRightsopenAccess
dc.subject.keywordCHUACes
dc.subject.keywordINIBICes
dc.typefidesArtículo Originales
dc.typesophosArtículo Originales
dc.volume.number11.es


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