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dc.contributor.authorBlasco Otero, Lucía
dc.contributor.authorAmbroa Abalo, Antón
dc.contributor.authorTRASTOY PENA, ROCIO 
dc.contributor.authorBleriot Rial, Ines Maria
dc.contributor.authorMoscoso Naya, Miriam
dc.contributor.authorFernández-Garcia, Laura
dc.contributor.authorPerez-Nadales, Elena
dc.contributor.authorFernández-Cuenca, Felipe
dc.contributor.authorTorre-Cisneros, Julian
dc.contributor.authorOteo-Iglesias, Jesus
dc.contributor.authorOliver, Antonio
dc.contributor.authorCanton, Rafael
dc.contributor.authorKidd, Tim
dc.contributor.authorNavarro, Ferran
dc.contributor.authorMiró, Elisenda
dc.contributor.authorPascual, Alvaro
dc.contributor.authorBou Arévalo, Germán 
dc.contributor.authorMartínez-Martínez, Luis
dc.contributor.authorTomás Carmona, María del Mar 
dc.date.accessioned2022-03-17T08:18:57Z
dc.date.available2022-03-17T08:18:57Z
dc.date.issued2020
dc.identifier.issn2045-2322
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/32346029es
dc.identifier.urihttp://hdl.handle.net/20.500.11940/16300
dc.description.abstractThe emergence of multidrug resistant (MDR) pathogenic bacteria is jeopardizing the value of antimicrobials, which had previously changed the course of medical science. In this study, we identified endolysins ElyA1 and ElyA2 (GH108-PG3 family), present in the genome of bacteriophages Ab1051Phi and Ab1052Phi, respectively. The muralytic activity of these endolysins against MDR clinical isolates (Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae) was tested using the turbidity reduction assay. Minimal inhibitory concentrations (MICs) of endolysin, colistin and a combination of endolysin and colistin were determined, and the antimicrobial activity of each treatment was confirmed by time kill curves. Endolysin ElyA1 displayed activity against all 25 strains of A. baumannii and P. aeruginosa tested and against 13 out of 17 strains of K. pneumoniae. Endolysin ElyA2 did not display any such activity. The combined antimicrobial activity of colistin and ElyA1 yielded a reduction in the colistin MIC for all strains studied, except K. pneumoniae. These results were confirmed in vivo in G. mellonella survival assays and in murine skin and lung infection models. In conclusion, combining colistin (1/4 MIC) with the new endolysin ElyA1 (350 microg) enhanced the bactericidal activity of colistin in both in vitro and in vivo studies. This will potentially enable reduction of the dose of colistin used in clinical practice.en
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshMicrobial Sensitivity Tests*
dc.subject.meshDrug Resistance*
dc.subject.meshColistin*
dc.subject.meshEndopeptidases*
dc.subject.meshGram-Negative Bacteria*
dc.titleIn vitro and in vivo efficacy of combinations of colistin and different endolysins against clinical strains of multi-drug resistant pathogensen
dc.typeJournal Articlees
dc.authorsophosBlasco, Lucia;Ambroa, Anton;Trastoy, Rocio;Bleriot, Ines;Moscoso, Miriam;Fernández-Garcia, Laura;Perez-Nadales, Elena;Fernández-Cuenca, Felipe;Torre-Cisneros, Julian;Oteo-Iglesias, Jesus;Oliver, Antonio;Canton, Rafael;Kidd, Tim;Navarro, Ferran;Miró, Elisenda;Pascual, Alvaro;Bou, German;Martínez-Martínez, Luis;Tomas, Maria
dc.identifier.doi10.1038/s41598-020-64145-7
dc.identifier.pmid32346029
dc.identifier.sophos36309
dc.issue.number1es
dc.journal.titleScientific Reportses
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario de A Coruña::Microbioloxíaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Biomédica da Coruña (INIBIC)es
dc.rights.accessRightsopenAccess
dc.subject.decspruebas de sensibilidad microbiana*
dc.subject.decsresistencia a medicamentos*
dc.subject.decsendopeptidasas*
dc.subject.decsbacterias gramnegativas*
dc.subject.decscolistina*
dc.subject.keywordCHUACes
dc.subject.keywordINIBICes
dc.typefidesArtículo Originales
dc.typesophosArtículo Originales
dc.volume.number10es


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