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dc.contributor.authorCasas Arozamena, Carlos
dc.contributor.authorDiaz, E.
dc.contributor.authorMoiola, C. P.
dc.contributor.authorAlonso-Alconada, L.
dc.contributor.authorFerreiros, A.
dc.contributor.authorAbalo Piñeiro, Alicia
dc.contributor.authorGil, C. L.
dc.contributor.authorOltra, S. S.
dc.contributor.authorde Santiago, J.
dc.contributor.authorCabrera, S.
dc.contributor.authorSampayo, V.
dc.contributor.authorBouso, M.
dc.contributor.authorARIAS BALTAR, MARIA EFIGENIA 
dc.contributor.authorCueva Bañuelos, Juan Fernando 
dc.contributor.authorColas, E.
dc.contributor.authorVilar Lagares, Ana
dc.contributor.authorGil-Moreno, A.
dc.contributor.authorAbal Posada, Miguel 
dc.contributor.authorMoreno-Bueno, G.
dc.contributor.authorMuinelo Romay , Laura
dc.date.accessioned2022-04-29T10:26:18Z
dc.date.available2022-04-29T10:26:18Z
dc.date.issued2020
dc.identifier.issn2077-0383
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/32098121es
dc.identifier.urihttp://hdl.handle.net/20.500.11940/16604
dc.description.abstractThe incidence and mortality of endometrial cancer (EC) have risen in recent years, hence more precise management is needed. Therefore, we combined different types of liquid biopsies to better characterize the genetic landscape of EC in a non-invasive and dynamic manner. Uterine aspirates (UAs) from 60 patients with EC were obtained during surgery and analyzed by next-generation sequencing (NGS). Blood samples, collected at surgery, were used for cell-free DNA (cfDNA) and circulating tumor cell (CTC) analyses. Finally, personalized therapies were tested in patient-derived xenografts (PDXs) generated from the UAs. NGS analyses revealed the presence of genetic alterations in 93% of the tumors. Circulating tumor DNA (ctDNA) was present in 41.2% of cases, mainly in patients with high-risk tumors, thus indicating a clear association with a more aggressive disease. Accordingly, the results obtained during the post-surgery follow-up indicated the presence of ctDNA in three patients with progressive disease. Moreover, 38.9% of patients were positive for CTCs at surgery. Finally, the efficacy of targeted therapies based on the UA-specific mutational landscape was demonstrated in PDX models. Our study indicates the potential clinical applicability of a personalized strategy based on a combination of different liquid biopsies to characterize and monitor tumor evolution, and to identify targeted therapies.en
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleGenomic Profiling of Uterine Aspirates and cfDNA as an Integrative Liquid Biopsy Strategy in Endometrial Canceren
dc.typeJournal Articlees
dc.authorsophosCasas-Arozamena, C.;Diaz, E.;Moiola, C. P.;Alonso-Alconada, L.;Ferreiros, A.;Abalo, A.;Gil, C. L.;Oltra, S. S.;de Santiago, J.;Cabrera, S.;Sampayo, V.;Bouso, M.;Arias, E.;Cueva, J.;Colas, E.;Vilar, A.;Gil-Moreno, A.;Abal, M.;Moreno-Bueno, G.;Muinelo-Romay, L.
dc.identifier.doi10.3390/jcm9020585
dc.identifier.pmid32098121
dc.identifier.sophos39570
dc.issue.number2es
dc.journal.titleJournal of Clinical Medicinees
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Anatomía Patolóxicaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Oncoloxía médicaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Xinecoloxía e Obstetriciaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS)es
dc.rights.accessRightsopenAccess
dc.subject.keywordCHUSes
dc.subject.keywordIDISes
dc.typefidesArtículo Originales
dc.typesophosArtículo Originales
dc.volume.number9es


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