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dc.contributor.authorCouselo Seijas, Marinela
dc.contributor.authorLópez Canoa, José Nicolas
dc.contributor.authorFernández González, Ángel Luís
dc.contributor.authorGonzález Melchor, Laila
dc.contributor.authorSeoane Camino, Luisa Maria 
dc.contributor.authorDurán Muñoz, Dario 
dc.contributor.authorRozados Luis, Adriana
dc.contributor.authorGonzález Juanatey, José Ramón 
dc.contributor.authorRodríguez Mañero, Moises 
dc.contributor.authorEiras Penas, Sonia
dc.date.accessioned2022-04-29T10:26:55Z
dc.date.available2022-04-29T10:26:55Z
dc.date.issued2020
dc.identifier.issn1582-1838
dc.identifier.otherhttps://www.ncbi.nlm.nih.gov/pubmed/32767737es
dc.identifier.urihttp://hdl.handle.net/20.500.11940/16613
dc.description.abstractThe modulation of acetylcholine (ACh) release by botulinum toxin injection into epicardial fat diminishes atrial fibrillation (AF) recurrence. These results suggest an interaction between autonomic imbalance and epicardial fat as risk factors of AF. Our aim was to study the inflammatory, lipidic and fibroblastic profile of epicardial stroma from patients who underwent open-heart surgery, their regulation by cholinergic activity and its association with AF. We performed in vitro and ex vivo assays from paired subcutaneous and epicardial stromal cells or explants from 33 patients. Acute ACh effects in inflammation and lipid-related genes were analysed by qPCR, in intracellular calcium mobilization were performed by Fluo-4 AM staining and in neutrophil migration by trans-well assays. Chronic ACh effects on lipid accumulation were visualized by AdipoRed. Plasma protein regulation by parasympathetic denervation was studied in vagotomized rats. Our results showed a higher pro-inflammatory profile in epicardial regarding subcutaneous stromal cells. Acute ACh treatment up-regulated monocyte chemoattractant protein 1 levels. Chronic ACh treatment improved lipid accumulation within epicardial stromal cells (60.50% [22.82-85.13] vs 13.85% [6.17-23.16], P < .001). Additionally, patients with AF had higher levels of fatty acid-binding protein 4 (1.54 +/- 0.01 vs 1.47 +/- 0.01, P = .005). Its plasma levels were pronouncedly declined in vagotomized rats (2.02 +/- 0.21 ng/mL vs 0.65 +/- 0.23 ng/mL, P < .001). Our findings support the characterization of acute or chronic cholinergic activity on epicardial stroma and its association with AF.en
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshAdipose Tissue*
dc.subject.meshRats*
dc.subject.meshVagotomy*
dc.subject.meshObesity*
dc.subject.meshChemokine CCL2*
dc.subject.meshStromal Cells*
dc.subject.meshAnimals*
dc.subject.meshParasympathetic Nervous System*
dc.subject.meshHumans*
dc.subject.meshCells*
dc.subject.meshChemotaxis*
dc.subject.meshFatty Acid-Binding Proteins*
dc.subject.meshCardiac Surgical Procedures*
dc.subject.meshInflammation*
dc.subject.meshPericardium*
dc.subject.meshMiddle Aged*
dc.subject.meshSubcutaneous Fat*
dc.subject.meshHL-60 Cells*
dc.subject.meshAdipocytes*
dc.subject.meshGene Expression Profiling*
dc.subject.meshCalcium Signaling*
dc.subject.meshAcetylcholine*
dc.subject.meshNeutrophils*
dc.subject.meshAtrial Fibrillation*
dc.subject.meshAged*
dc.titleInflammatory and lipid regulation by cholinergic activity in epicardial stromal cells from patients who underwent open-heart surgeryen
dc.typeJournal Articlees
dc.authorsophosCouselo-Seijas, M.;Lopez-Canoa, J. N.;Fernandez, A. L.;Gonzalez-Melchor, L.;Seoane, L. M.;Duran-Munoz, D.;Rozados-Luis, A.;Gonzalez-Juanatey, J. R.;Rodriguez-Manero, M.;Eiras, S.
dc.identifier.doi10.1111/jcmm.15727
dc.identifier.pmid32767737
dc.identifier.sophos39620
dc.issue.number18es
dc.journal.titleJOURNAL OF CELLULAR AND MOLECULAR MEDICINEes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Cardioloxíaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Cirurxía Cardíacaes
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS)es
dc.page.initial10958es
dc.page.final10969es
dc.rights.accessRightsopenAccess
dc.subject.decsanimales*
dc.subject.decsproteínas de unión a ácidos grasos*
dc.subject.decsmediana edad*
dc.subject.decsgrasa subcutánea*
dc.subject.decstejido adiposo*
dc.subject.decsinflamación*
dc.subject.decspericardio*
dc.subject.decscélulas del estroma*
dc.subject.decsratas*
dc.subject.decscélulas HL-60*
dc.subject.decsseñalización por calcio*
dc.subject.decsprocedimientos quirúrgicos cardíacos*
dc.subject.decsquimiotaxis*
dc.subject.decsadipocitos*
dc.subject.decsanciano*
dc.subject.decsobesidad*
dc.subject.decshumanos*
dc.subject.decscélulas*
dc.subject.decsacetilcolina*
dc.subject.decsfibrilación atrial*
dc.subject.decsperfiles de expresión génica*
dc.subject.decssistema nervioso parasimpático*
dc.subject.decsquimiocina CCL2*
dc.subject.decsvagotomía*
dc.subject.decsneutrófilos*
dc.subject.keywordCHUSes
dc.subject.keywordIDISes
dc.typefidesArtículo Originales
dc.typesophosArtículo Originales
dc.volume.number24es


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Atribución 4.0 Internacional
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