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dc.contributor.authorRodriguez-Temporal, D.
dc.contributor.authorAlcaide, F.
dc.contributor.authorMareković, I.
dc.contributor.authorO’Connor, J.A.
dc.contributor.authorGorton, R.
dc.contributor.authorvan Ingen, J.
dc.contributor.authorVan den Bossche, A.
dc.contributor.authorHéry-Arnaud, G.
dc.contributor.authorBeauruelle, C.
dc.contributor.authorOrth-Höller, D.
dc.contributor.authorPalacios-Gutiérrez, J.-J.
dc.contributor.authorTudó, G.
dc.contributor.authorBou Arévalo, Germán 
dc.contributor.authorCeyssens, P.-J.
dc.contributor.authorGarrigó, M.
dc.contributor.authorGonzález-Martin, J.
dc.contributor.authorGreub, G.
dc.contributor.authorHrabak, J.
dc.contributor.authorIngebretsen, A.
dc.contributor.authorMediavilla-Gradolph, M.C.
dc.contributor.authorOviaño García, Marina 
dc.contributor.authorPalop, B.
dc.contributor.authorPranada, A.B.
dc.contributor.authorQuiroga, L.
dc.contributor.authorRuiz-Serrano, M.J.
dc.contributor.authorRodríguez-Sánchez, B.
dc.date.accessioned2025-02-19T12:26:11Z
dc.date.available2025-02-19T12:26:11Z
dc.date.issued2022
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/20.500.11940/19571
dc.description.abstractThe ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2–98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
dc.language.isoenes
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleMulticentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
dc.typeJournal Articlees
dcterms.bibliographicCitationRodriguez-Temporal D, Alcaide F, Mareković I, O’Connor JA, Gorton R, van Ingen J, et al. Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification. Scientific Reports. 2022;12(1).
dc.authorsophosRodriguez-Temporal, B. D.;Alcaide, F.;Mareković, I.;O’Connor, J. A.;Gorton, R.;van Ingen, J.;Van den Bossche, A.;Héry-Arnaud, G.;Beauruelle, C.;Orth-Höller, D.;Palacios-Gutiérrez, J. J.;Tudó, G.;Bou, G.;Ceyssens, P. J.;Garrigó, M.;González-Martin, J.;Greub, G.;Hrabak, J.;Ingebretsen, A.;Mediavilla-Gradolph, M. C.;Oviaño, M.;Palop, B.;Pranada, A. B.;Quiroga, L.;Ruiz-Serrano, M. J.;Rodríguez, Sánchez
dc.identifier.doi10.1038/S41598-022-05315-7
dc.identifier.sophos61ff0a3413638e1cfc27a559
dc.issue.number1
dc.journal.titleScientific Reports
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-022-05315-7.pdfes
dc.rights.accessRightsopenAccess
dc.subject.keywordAS Coruñaes
dc.subject.keywordCHUACes
dc.subject.keywordINIBICes
dc.volume.number12


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