Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4
Feijoo Bandin, Sandra; Rodríguez Penas, Diego; García Rua, Vanessa; Mosquera Leal, Ana; Otero Santiago, Manuel Francisco; Pereira Lopez, Eva Maria; Rubio Álvarez, José; Martínez Soto, María Isabel; Seoane Camino, Luisa Maria; Gualillo ., Oreste; Calaza Cabanas, Manuel; García-Caballero Parada, Tomas; Portolés, M.; Roselló-Lletí, E.; Diéguez González, Carlos; Rivera, M.; González Juanatey, José Ramón; Lago Paz, Francisca
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Identificadores![](/xmlui/themes/Mirage2//images/despl_identif.png)
Identificadores
URI: http://hdl.handle.net/20.500.11940/1982
PMID: 24064358
DOI: 10.1210/en.2013-1497
ISSN: 0013-7227
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Fecha de publicación
2013Título de revista
Endocrinology
Tipo de contenido
Artigo
MeSH
Animal Feed | Animals | Calcium-Binding Proteins | Cells, Cultured | DNA-Binding Proteins | Diet | Dietary Fats/pharmacology | Female | Gene Expression Regulation | Glucose | Glucose Transporter Type 4 | Humans | Male | Mice | Myocytes, Cardiac | Nerve Tissue Proteins | Nucleobindins | RNA, Messenger | Rats | Rats, Sprague-DawleyResumen
Nesfatin-1, a satiety-inducing peptide identified in hypothalamic regions that regulate energy balance, is an integral regulator of energy homeostasis and a putative glucose-dependent insulin coadjuvant. We investigated its production by human cardiomyocytes and its effects on glucose uptake, in the main cardiac glucose transporter GLUT-4 and in intracellular signaling. Quantitative RT-PCR, Western blots, confocal immunofluorescence microscopy, and ELISA of human and murine cardiomyocytes and/or cardiac tissue showed that cardiomyocytes can synthesize and secrete nesfatin-1. Confocal microscopy of cultured cardiomyocytes after GLUT-4 labeling showed that nesfatin-1 mobilizes this glucose transporter to cell peripherals. The rate of 2-deoxy-D-[(3)H]glucose incorporation demonstrated that nesfatin-1 induces glucose uptake by HL-1 cells and cultured cardiomyocytes. Nesfatin-1 induced dose- and time-dependent increases in the phosphorylation of ERK1/2, AKT, and AS160. In murine and human cardiac tissue, nesfatin-1 levels varied with diet and coronary health. In conclusion, human and murine cardiomyocytes can synthesize and secrete nesfatin-1, which is able to induce glucose uptake and the mobilization of the glucose transporter GLUT-4 in these cells. Nesfatin-1 cardiac levels are regulated by diet and coronary health.
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