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dc.contributor.authorDávila-Rodríguez, MI
dc.contributor.authorCortés Gutiérrez, EI
dc.contributor.authorCerda Flores, RM
dc.contributor.authorPita, M
dc.contributor.authorFernández García, José Luis 
dc.contributor.authorLópez Fernández, C
dc.contributor.authorGosálvez, J
dc.date.accessioned2017-06-07T07:07:04Z
dc.date.available2017-06-07T07:07:04Z
dc.date.issued2011
dc.identifier.citationDávila-Rodríguez MI, Cortés Gutiérrez EI, Cerda Flores RM, Pita M, Fernández JL, López-Fernández C, Gosálvez J. Constitutive heterochromatin polymorphisms in human chromosomes identified by whole comparative genomic hybridization. Eur J Histochem. 2011;55(3):e28. doi: 10.4081/ejh.2011.e28. Epub 2011 Aug 27. PMID: 22073375; PMCID: PMC3203469.
dc.identifier.issn1121-760X
dc.identifier.urihttp://hdl.handle.net/20.500.11940/2796
dc.description.abstractWhole comparative genomic hybridization (W-CGH) is a new technique that reveals cryptic differences in highly repetitive DNA sequences, when different genomes are compared using metaphase or interphase chromosomes. W-CGH provides a quick approach to identify differential expansion of these DNA sequences at the single-chromosome level in the whole genome. In this study, we have determined the frequency of constitutive chromatin polymorphisms in the centromeric regions of human chromosomes using a whole-genome in situ cross-hybridization method to compare the whole genome of five different unrelated individuals. Results showed that the pericentromeric constitutive heterochromatin of chromosome 6 exhibited a high incidence of polymorphisms in repetitive DNA families located in pericentromeric regions. The constitutive heterochromatin of chromosomes 5 and 9 was also identified as highly polymorphic. Although further studies are necessary to corroborate and assess the overall incidence of these polymorphisms in human populations, the use of W-CGH could be pertinent and of clinical relevance to assess rapidly, from a chromosomal viewpoint, genome similarities and differences in closely related genomes such as those of relatives, or in more specific situations such as bone marrow transplantation where chimerism is produced in the recipient.
dc.language.isoeng
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshChromosomes, Human 
dc.subject.meshComparative Genomic Hybridization 
dc.subject.meshGenome, Human 
dc.subject.meshHeterochromatin 
dc.subject.meshPolymorphism, Genetic
dc.titleConstitutive heterochromatin polymorphisms in human chromosomes identified by whole comparative genomic hybridization
dc.typeArtigoes
dc.authorsophosDavila-Rodriguez, M. I.
dc.authorsophosGutierrez, E. I. C.
dc.authorsophosFlores, R. M. C.
dc.authorsophosPita, M.
dc.authorsophosFernandez, J. L.
dc.authorsophosLopez-Fernandez, C.
dc.authorsophosGosalvez, J.
dc.identifier.doi10.4081/ejh.2011.e28
dc.identifier.pmid22073375
dc.identifier.sophos9071
dc.issue.number3
dc.journal.titleEUROPEAN JOURNAL OF HISTOCHEMISTRY
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña::INIBIC.- Instituto de Investigación Biomédica
dc.page.initial151
dc.page.final155
dc.rights.accessRightsopenAccess
dc.subject.decsCromosomas Humanos
dc.subject.decsHibridación Genómica Comparativa
dc.subject.decsGenoma Humano
dc.subject.decsHeterocromatina 
dc.subject.decsPolimorfismo Genético 
dc.typesophosArtículo Original
dc.volume.number55


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