Mostrar el registro sencillo del ítem

dc.contributor.authorBLANCO GARCIA, FRANCISCO JAVIER 
dc.contributor.authorFernández García, José Luis 
dc.contributor.authorRego Pérez, Ignacio 
dc.contributor.authorFernández Moreno, Mercedes
dc.contributor.authorTamayo Novas, María
dc.contributor.authorMosquera Rey, Alejandro 
dc.contributor.authorSoto Hermida, Angel
dc.contributor.authorRego Pérez, Ignacio 
dc.contributor.authorBLANCO GARCIA, FRANCISCO JAVIER 
dc.date.accessioned2017-06-07T07:07:23Z
dc.date.available2017-06-07T07:07:23Z
dc.date.issued2011
dc.identifier.issn1471-2474
dc.identifier.urihttp://hdl.handle.net/20.500.11940/2845
dc.description.abstractBACKGROUND: Oxidative stress due to the overproduction of nitric oxide (NO) and other oxygen reactive species (ROS), play a main role in the initiation and progression of the OA disease and leads to the degeneration of mitochondria. Therefore, the goal of this work is to describe the difference in telomere length of peripheral blood leukocytes (PBLs) and Nitric Oxide (NO) production between mitochondrial DNA (mtDNA) haplogroup J and non-J carriers, as indirect approaches of oxidative stress. METHODS: The telomere length of PBL was analyzed in DNA samples from 166 healthy controls (114 J and 52 non-J) and 79 OA patients (41 J and 38 non-J) by means of a validated qPCR method. The NO production was assessed in 7 carriers of the haplogroup J and 27 non-J carriers, by means of the colorimetric reaction of the Griess reagent in supernatants of cultured chondrocytes. Inducible nitric oxide synthase (iNOS) mRNA from these samples was analyzed by qPCR. Appropiated statistical analyses were performed RESULTS: Carriers of the haplogroup J showed a significantly longer telomere length of PBLs than non-J carriers, regardless of age, gender and diagnosis (p = 0.025). Cultured chondrocytes carrying the mtDNA haplogroup J also showed a lower NO production than non-J carriers (p = 0.043). No significant correlations between age and telomore length of PBLs were detected neither for carriers of the haplogroup J nor for non-J carriers. A strong positive correlation between NO production and iNOS expression was also observed (correlation coefficient = 0.791, p < 0.001). CONCLUSION: The protective effect of the mtDNA haplogroup J in the OA disease arise from a lower oxidative stress in carriers of this haplogroup, since this haplogroup is related to lower NO production and hence longer telomere length of PBLs too.
dc.language.isoeng
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titlemtDNA haplogroup J modulates telomere length and nitric oxide production
dc.typeArtigoes
dc.authorsophosFernandez-Moreno, M.
dc.authorsophosTamayo, M.
dc.authorsophosSoto-Hermida, A.
dc.authorsophosMosquera, A.
dc.authorsophosOreiro, N.
dc.authorsophosFernandez-Lopez, C.
dc.authorsophosFernandez, J. L.
dc.authorsophosRego-Perez, I.
dc.authorsophosBlanco, F. J.
dc.identifier.doi10.1186/1471-2474-12-283
dc.identifier.pmid22171676
dc.identifier.sophos9117
dc.journal.titleBMC MUSCULOSKELETAL DISORDERS
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario A Coruña::Reumatoloxía
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña::INIBIC.- Instituto de Investigación Biomédica
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario A Coruña::Xenética
dc.page.initial283
dc.rights.accessRightsopenAccess
dc.typesophosArtículo Original
dc.volume.number12


Ficheros en el ítem

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como Atribución 4.0 Internacional