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dc.contributor.authorRodriguez Rigueiro, Teresa
dc.contributor.authorValladares Ayerbes, Manuel 
dc.contributor.authorHaz Conde, María del Mar 
dc.contributor.authorBlanco Calvo, Moisés 
dc.contributor.authorAparicio Gallego, Guadalupe
dc.contributor.authorFernández Puente, Patricia 
dc.contributor.authorBLANCO GARCIA, FRANCISCO JAVIER 
dc.contributor.authorAparicio Gallego, Guadalupe
dc.contributor.authorLorenzo Lorenzo, María Jesus 
dc.contributor.authorAntón Aparicio, Luis Miguel
dc.contributor.authorFigueroa, Angélica
dc.date.accessioned2017-06-07T07:08:52Z
dc.date.available2017-06-07T07:08:52Z
dc.date.issued2011
dc.identifier.issn1615-9853
dc.identifier.urihttp://hdl.handle.net/20.500.11940/3116
dc.description.abstractMost of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry in a clinical routine as it is difficult to recover intact protein from these FFPE tissues. Here, we report a novel, short time-consuming and cost-effective method to extract full-length, non-degraded proteins from FFPE tissues. This procedure is combined with an effective and non-toxic deparaffinisation process and an extraction method based on antigen-retrieval, high concentration of SDS and high temperature. We have obtained enough intact protein to be detected by Western blotting analysis. This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases.
dc.language.isoeng
dc.titleA novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues
dc.typeArtigoes
dc.authorsophosRodríguez-Rigueiro, T.
dc.authorsophosValladares-Ayerbes, M.
dc.authorsophosHaz-Conde, M.
dc.authorsophosBlanco, M.
dc.authorsophosAparicio, G.
dc.authorsophosFernández-Puente, P.
dc.authorsophosBlanco, F. J.
dc.authorsophosLorenzo, M. J.
dc.authorsophosAparicio, L. A.
dc.authorsophosFigueroa, A.
dc.identifier.doi10.1002/pmic.201000809
dc.identifier.pmid21591256
dc.identifier.sophos9397
dc.issue.number12
dc.journal.titlePROTEOMICS
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario A Coruña::Oncoloxía médica
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña::INIBIC.- Instituto de Investigación Biomédica
dc.page.initial2555
dc.page.final2559
dc.rights.accessRightsopenAccess
dc.typesophosArtículo Original
dc.volume.number11


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