RUNA digital repository

    • Español
    • Galego
    • English
  • English 
    • Español
    • Galego
    • English
  • Login
RUNABibliosaúdeXunta de galicia. Consellería de sanidadeServicio Galego de saúde
  • REPOSITORY
  • ABOUT US
    • About RUNA
    • Normative
    • Sergas Policy
  • HELP
    • Help
    • FAQ
  •   RUNA Home
  • Scientific publication
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Two-dimensional proteome reference map of Vibrio tapetis, the aetiological agent of brown ring disease in clams

Bermúdez-Crespo, J; Balboa, S; Alonso Lorenzo, Jana; Romalde, J L;
Thumbnail
Statistics
Statistics
View Usage Statistics
Identifiers
Identifiers
URI: http://hdl.handle.net/20.500.11940/4952
PMID: 22372382
DOI: 10.1111/j.1365-2672.2012.05271.x
ISSN: 1364-5072
Full record
Services
Services
RISMendeleyLinksolver
Files view or download
Files view or download
J Appl Microbiol . 2012 May;112(5):853-64 (952.0Kb)
Date issued
2012
Journal title
JOURNAL OF APPLIED MICROBIOLOGY
Type of content
Artigo
Abstract
Aims: Vibrio tapetis is the etiological agent of brown ring disease (BRD) in clams, one of the most threatening diseases affecting this commercially important bivalve. In this study we have constructed a proteome reference map of the V. tapetis type strain CECT 4600T. Methods and Results: Eighty-two proteins, consistently present in all 2D-gels, were identified by mass spectrometry or by de novo sequencing. The majority of the proteins identified (66%) belonged to four COG categories: 'Carbohydrate transport and metabolism', 'Post-translational modification, protein turnover and chaperones', 'Energy production', and 'Amino acid transport and metabolism'. Glyceraldehyde-3-phosphate dehydrogenase, enolase, fructose-bisphosphate aldolase, phosphoglycerate kinase. molecular chaperones Dnak and GroEL, alkyl hydroperoxide reductase, peptidyl-prolyl cis-trans isomerase B and factor Tu, were identified among the 20 most abundant proteins. A comparison of this reference map with that obtained for the V. tapetis strain GR0202RD, with different origin and pathophysiological characteristics, was performed. Conclusions: Under the culture conditions employed in this study, glucose degradation is one of the major pathways for energy production in Vibrio tapetis. In addition, the two strains studied, although with remarkable differences at genetic and pathophysiological levels, showed a high similarity under laboratory conditions. Significance and Impact of the Study: The results obtained here can be considered as a first step to gather valuable information on protein expression, related not only to diverse cellular functions and regulation but also to pathogenesis and bacterium-host interactions in the disease process.

Browse

All of RUNACollectionsCentersAuthorsTitlesDeCSMeSHCIETypes of contentThis CollectionCentersAuthorsTitlesDeCSMeSHCIETypes of content

Statistics

View Usage Statistics

OF INTEREST

About Open AccessCopyright
TwitterRSS
Xunta de Galicia
© Xunta de Galicia. Información mantida e publicada na internet pola Consellería de Sanidade o Servizo Galego de Saúde
Legal warning | RSS
Galicia