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dc.contributor.authorGurriarán Rodríguez, Uxía
dc.contributor.authorAL-MASSADI IGLESIAS, OMAR 
dc.contributor.authorCrujeiras Martínez, Ana Belén
dc.contributor.authorSeoane Mosteiro, Carlos 
dc.contributor.authorAmil-Diz, María
dc.contributor.authorBeiroa Tarrío, Daniel
dc.contributor.authorNogueiras Pozo, Rubén
dc.contributor.authorSeoane Camino, Luisa Maria 
dc.contributor.authorGallego, Rosalía
dc.contributor.authorPazos Randulfe, Yolanda 
dc.contributor.authorCasanueva Freijo, Felipe 
dc.contributor.authorPérez Camiña, Jesús 
dc.date.accessioned2017-06-07T07:21:36Z
dc.date.available2017-06-07T07:21:36Z
dc.date.issued2011
dc.identifier.issn0022-0795
dc.identifier.urihttp://hdl.handle.net/20.500.11940/5528
dc.description.abstractThis study aimed to investigate the role of preproghrelin-derived peptides in adipogenesis. Immunocytochemical analysis of 3T3-L1 adipocyte cells showed stronger preproghrelin expression compared with that observed in 3T3-L1 preadipocyte cells. Insulin promoted this expression throughout adipogenesis identifying mTORC1 as a critical downstream substrate for this profile. The role of preproghrelin-derived peptides on the differentiation process was supported by preproghrelin knockdown experiments, which revealed its contribution to adipogenesis. Neutralization of endogenous O-acyl ghrelin (acylated ghrelin), unacylated ghrelin, and obestatin by specific antibodies supported their adipogenic potential. Furthermore, a parallel increase in the expression of ghrelin-associated enzymatic machinery, prohormone convertase 1/3 (PC1/3) and membrane-bound O-acyltransferase 4 (MBOAT4), was dependent on the expression of preproghrelin in the course of insulin-induced adipogenesis. The coexpression of preproghrelin system and their receptors, GHSR1a and GPR39, during adipogenesis supports an autocrine/paracrine role for these peptides. Preproghrelin, PC1/3, and MBOAT4 exhibited dissimilar expression depending on the white fat depot, revealing their regulation in a positive energy balance situation in mice. The results underscore a key role for preproghrelin-derived peptides on adipogenesis through an autocrine/paracrine mechanism.
dc.language.isoeng
dc.subject.meshMale
dc.subject.meshAnimals
dc.subject.meshMice
dc.subject.meshInsulin
dc.subject.mesh3T3-L1 Cells
dc.subject.meshRNA, Small Interfering
dc.subject.meshAdipocytes
dc.subject.meshAdipogenesis
dc.subject.meshPeptide Hormones
dc.subject.meshProtein Precursors
dc.titlePreproghrelin expression is a key target for insulin action on adipogenesis.
dc.typeArtigoes
dc.authorsophosGurriarán-Rodríguez, Uxía
dc.authorsophosAl-Massadi, Omar
dc.authorsophosCrujeiras, Ana Belén
dc.authorsophosMosteiro, Carlos S
dc.authorsophosAmil-Diz, María
dc.authorsophosBeiroa, Daniel
dc.authorsophosNogueiras, Rubén
dc.authorsophosSeoane, Luisa María
dc.authorsophosGallego, Rosalía
dc.authorsophosPazos, Yolanda
dc.authorsophosCasanueva, Felipe F
dc.authorsophosCamiña, Jesús P
dc.identifier.doi10.1530/JOE-11-0233
dc.identifier.isi292764400001
dc.identifier.pmid21690170
dc.identifier.sophos9882
dc.issue.number2
dc.journal.titleJOURNAL OF ENDOCRINOLOGY
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago - Complexo Hospitalario Universitario de Santiago::Endocrinoloxía
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago::IDIS.- Instituto de investigaciones sanitarias de Santiago
dc.page.initialR1
dc.page.final7
dc.rights.accessRightsopenAccess
dc.typesophosArtículo Original
dc.volume.number210


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