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Preproghrelin expression is a key target for insulin action on adipogenesis.
dc.contributor.author | Gurriarán Rodríguez, Uxía | |
dc.contributor.author | AL-MASSADI IGLESIAS, OMAR | |
dc.contributor.author | Crujeiras Martínez, Ana Belén | |
dc.contributor.author | Seoane Mosteiro, Carlos | |
dc.contributor.author | Amil-Diz, María | |
dc.contributor.author | Beiroa Tarrío, Daniel | |
dc.contributor.author | Nogueiras Pozo, Rubén | |
dc.contributor.author | Seoane Camino, Luisa Maria | |
dc.contributor.author | Gallego, Rosalía | |
dc.contributor.author | Pazos Randulfe, Yolanda | |
dc.contributor.author | Casanueva Freijo, Felipe | |
dc.contributor.author | Pérez Camiña, Jesús | |
dc.date.accessioned | 2017-06-07T07:21:36Z | |
dc.date.available | 2017-06-07T07:21:36Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 0022-0795 | |
dc.identifier.uri | http://hdl.handle.net/20.500.11940/5528 | |
dc.description.abstract | This study aimed to investigate the role of preproghrelin-derived peptides in adipogenesis. Immunocytochemical analysis of 3T3-L1 adipocyte cells showed stronger preproghrelin expression compared with that observed in 3T3-L1 preadipocyte cells. Insulin promoted this expression throughout adipogenesis identifying mTORC1 as a critical downstream substrate for this profile. The role of preproghrelin-derived peptides on the differentiation process was supported by preproghrelin knockdown experiments, which revealed its contribution to adipogenesis. Neutralization of endogenous O-acyl ghrelin (acylated ghrelin), unacylated ghrelin, and obestatin by specific antibodies supported their adipogenic potential. Furthermore, a parallel increase in the expression of ghrelin-associated enzymatic machinery, prohormone convertase 1/3 (PC1/3) and membrane-bound O-acyltransferase 4 (MBOAT4), was dependent on the expression of preproghrelin in the course of insulin-induced adipogenesis. The coexpression of preproghrelin system and their receptors, GHSR1a and GPR39, during adipogenesis supports an autocrine/paracrine role for these peptides. Preproghrelin, PC1/3, and MBOAT4 exhibited dissimilar expression depending on the white fat depot, revealing their regulation in a positive energy balance situation in mice. The results underscore a key role for preproghrelin-derived peptides on adipogenesis through an autocrine/paracrine mechanism. | |
dc.language.iso | eng | |
dc.subject.mesh | Male | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Mice | |
dc.subject.mesh | Insulin | |
dc.subject.mesh | 3T3-L1 Cells | |
dc.subject.mesh | RNA, Small Interfering | |
dc.subject.mesh | Adipocytes | |
dc.subject.mesh | Adipogenesis | |
dc.subject.mesh | Peptide Hormones | |
dc.subject.mesh | Protein Precursors | |
dc.title | Preproghrelin expression is a key target for insulin action on adipogenesis. | |
dc.type | Artigo | es |
dc.authorsophos | Gurriarán-Rodríguez, Uxía | |
dc.authorsophos | Al-Massadi, Omar | |
dc.authorsophos | Crujeiras, Ana Belén | |
dc.authorsophos | Mosteiro, Carlos S | |
dc.authorsophos | Amil-Diz, María | |
dc.authorsophos | Beiroa, Daniel | |
dc.authorsophos | Nogueiras, Rubén | |
dc.authorsophos | Seoane, Luisa María | |
dc.authorsophos | Gallego, Rosalía | |
dc.authorsophos | Pazos, Yolanda | |
dc.authorsophos | Casanueva, Felipe F | |
dc.authorsophos | Camiña, Jesús P | |
dc.identifier.doi | 10.1530/JOE-11-0233 | |
dc.identifier.isi | 292764400001 | |
dc.identifier.pmid | 21690170 | |
dc.identifier.sophos | 9882 | |
dc.issue.number | 2 | |
dc.journal.title | JOURNAL OF ENDOCRINOLOGY | |
dc.organization | Servizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago - Complexo Hospitalario Universitario de Santiago::Endocrinoloxía | |
dc.organization | Servizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago::IDIS.- Instituto de investigaciones sanitarias de Santiago | |
dc.page.initial | R1 | |
dc.page.final | 7 | |
dc.rights.accessRights | openAccess | |
dc.typesophos | Artículo Original | |
dc.volume.number | 210 |