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dc.contributor.authorGurriarán Rodríguez, Uxía
dc.contributor.authorAl-Massadi Iglesias, Omar
dc.contributor.authorRoca Rivada, Arturo
dc.contributor.authorCrujeiras Martínez, Ana Belén
dc.contributor.authorGallego, Rosalía
dc.contributor.authorPardo Pérez, María 
dc.contributor.authorSeoane Camino, Luisa Maria 
dc.contributor.authorPazos Randulfe, Yolanda 
dc.contributor.authorCasanueva Freijo, Felipe 
dc.contributor.authorPérez Camiña, Jesús 
dc.date.accessioned2017-06-07T07:22:00Z
dc.date.available2017-06-07T07:22:00Z
dc.date.issued2011
dc.identifier.issn1582-1838
dc.identifier.urihttp://hdl.handle.net/20.500.11940/5592
dc.description.abstractThe role of obestatin, a 23-amino-acid peptide encoded by the ghrelin gene, on the control of the metabolism of pre-adipocyte and adipocytes as well as on adipogenesis was determined. For in vitro assays, pre-adipocyte and adipocyte 3T3-L1 cells were used to assess the obestatin effect on cell metabolism and adipogenesis based on the regulation of the key enzymatic nodes, Akt and AMPK and their downstream targets. For in vivo assays, white adipose tissue (WAT) was obtained from male rats under continuous subcutaneous infusion of obestatin. Obestatin activated Akt and its downstream targets, GSK3alpha/beta, mTOR and S6K1, in 3T3-L1 adipocyte cells. Simultaneously, obestatin inactivated AMPK in this cell model. In keeping with this, ACC phosphorylation was also decreased. This fact was confirmed in vivo in white adipose tissue (omental, subcutaneous and gonadal) obtained from male rats under continuous sc infusion of obestatin (24 and 72 hrs). The relevance of obestatin as regulator of adipocyte metabolism was supported by AS160 phosphorylation, GLUT4 translocation and augment of glucose uptake in 3T3-L1 adipocyte cells. In contrast, obestatin failed to modify translocation of fatty acid transporters, FATP1, FATP4 and FAT/CD36, to plasma membrane. Obestatin treatment in combination with IBMX and DEX showed to regulate the expression of C/EBPalpha, C/EBPbeta, C/EBPdelta and PPARgamma promoting adipogenesis. Remarkable, preproghrelin expression, and thus obestatin expression, increased during adipogenesis being sustained throughout terminal differentiation. Neutralization of endogenous obestatin secreted by 3T3-L1 cells by anti-obestatin antibody decreased adipocyte differentiation. Furthermore, knockdown experiments by preproghrelin siRNA supported that obestatin contributes to adipogenesis. In summary, obestatin promotes adipogenesis in an autocrine/paracrine manner, being a regulator of adipocyte metabolism. These data point to a putative role in the pathogenesis of metabolic syndrome.
dc.language.isoeng
dc.titleObestatin as a regulator of adipocyte metabolism and adipogenesis
dc.typeArtigoes
dc.authorsophosGurriaran-Rodriguez, Uxia
dc.authorsophosAl-Massadi, Omar
dc.authorsophosRoca-Rivada, Arturo
dc.authorsophosBelen Crujeiras, Ana
dc.authorsophosGallego, Rosalia
dc.authorsophosPardo, Maria
dc.authorsophosMaria Seoane, Luisa
dc.authorsophosPazos, Yolanda
dc.authorsophosCasanueva, Felipe F.
dc.authorsophosCamina, Jesus P.
dc.identifier.doi10.1111/j.1582-4934.2010.01192.x
dc.identifier.isi294597400012
dc.identifier.pmid21029370
dc.identifier.sophos10071
dc.issue.number9
dc.journal.titleJOURNAL OF CELLULAR AND MOLECULAR MEDICINE
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago - Complexo Hospitalario Universitario de Santiago::Endocrinoloxía
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago::IDIS.- Instituto de investigaciones sanitarias de Santiago
dc.page.initial1927
dc.page.final1940
dc.rights.accessRightsopenAccess
dc.typesophosArtículo Original
dc.volume.number15


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