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dc.contributor.authorValladares Ayerbes, Manuel 
dc.contributor.authorReboredo Lopez, Margarita 
dc.contributor.authorMedina Villaamil, Vanessa
dc.contributor.authorIglesias Díaz, Pilar 
dc.contributor.authorLorenzo Patiño, María José 
dc.contributor.authorHaz Conde, María del Mar 
dc.contributor.authorSantamarina Cainzos, Isabel
dc.contributor.authorBlanco Calvo, Moisés 
dc.contributor.authorFernández Tajes, Juan
dc.contributor.authorQuindós Varela, María 
dc.contributor.authorCarral Maseda, Alberto 
dc.contributor.authorFigueroa Conde-Valvís, Angélica
dc.contributor.authorAntón Aparicio, Luis Miguel
dc.contributor.authorCalvo Martínez, Lourdes 
dc.date.accessioned2017-06-07T07:32:58Z
dc.date.available2017-06-07T07:32:58Z
dc.date.issued2012
dc.identifier.citationValladares-Ayerbes M, Reboredo M, Medina-Villaamil V, Iglesias-Díaz P, Lorenzo-Patiño MJ, Haz M, Santamarina I, Blanco M, Fernández-Tajes J, Quindós M, Carral A, Figueroa A, Antón-Aparicio LM, Calvo L. Circulating miR-200c as a diagnostic and prognostic biomarker for gastric cancer. J Transl Med. 2012 Sep 6;10:186. doi: 10.1186/1479-5876-10-186. PMID: 22954417; PMCID: PMC3494541.
dc.identifier.issn1479-5876
dc.identifier.urihttp://hdl.handle.net/20.500.11940/7804
dc.description.abstractBackground: MicroRNAs are aberrantly expressed and correlate with tumourigenesis and the progression of solid tumours. The miR-200 family determines the epithelial phenotype of cancer cells and regulates invasiveness and migration. Thus, we hypothesised that the quantitative detection of the miR-200 family as epithelial-specific microRNAs in the blood could be a useful clinical biomarker for gastric cancer (GC). Methods: We initially validated the expression levels of miR-200a, 200b, 200c and 141 in GC cell lines (n = 2) and blood from healthy controls (n = 19) using real-time quantitative reverse transcription PCR (qRT-PCR). The microarray expression profiles of the miR-200 family in 160 paired samples of non-tumour gastric mucosae and GC were downloaded through ArrayExpress and analysed. MiR-200c was selected for clinical validation. The qRT-PCR prospective assessment of miR-200c was performed using 67 blood samples (52 stage I-IV GC patients and 15 controls); the area under the receiver operating characteristic curve (AUC-ROC) was estimated. The Kaplan-Meier and Breslow-Wilcoxon tests were used to assess the correlation of miR-200c with overall and progression-free survival (OS and PFS). Multivariate analyses were performed using the Cox model. Results: The miR-200c blood expression levels in GC patients were significantly higher than in normal controls (p = 0.018). The AUC-ROC was 0.715 (p = 0.012). The sensitivity, specificity and accuracy rates of 65.4%, 100% and 73.1%, respectively, were observed. The levels of miR-200c in the blood above the cutoff defined by the ROC curve was found in 17.6% of stage I-II GC patients, 20.6% of stage III patients and 67.7% of stage IV patients (p < 0.001). The miR-200c expression levels were not associated with clinical or pathological characteristics or recent surgical procedures. There was a correlation (p = 0.016) with the number of lymph node metastases and the increased expression levels of miR-200c in blood were significantly associated with a poor OS (median OS, 9 vs 24 months; p = 0.016) and PFS (median PFS, 4 vs 11 months; p = 0.044). Multivariate analyses confirmed that the upregulation of miR-200c in the blood was associated with OS (HR = 2.24; p = 0.028) and PFS (HR = 2.27; p = 0.028), independent of clinical covariates. Conclusions: These data suggest that increased miR-200c levels are detected in the blood of gastric cancer patients. MiR-200c has the potential to be a predictor of progression and survival.
dc.language.isoeng
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshBiomarkers, Tumor
dc.subject.meshArea Under Curve 
dc.subject.meshMicroRNAs 
dc.subject.meshPolymerase Chain Reaction 
dc.subject.meshStomach Neoplasms 
dc.titleCirculating miR-200c as a diagnostic and prognostic biomarker for gastric cancer
dc.typeArtigoes
dc.authorsophosValladares-Ayerbes, M.
dc.authorsophosReboredo, M.
dc.authorsophosMedina-Villaamil, V.
dc.authorsophosIglesias-Diaz, P.
dc.authorsophosLorenzo-Patino, M. J.
dc.authorsophosHaz, M.
dc.authorsophosSantamarina, I.
dc.authorsophosBlanco, M.
dc.authorsophosFernandez-Tajes, J.
dc.authorsophosQuindos, M.
dc.authorsophosCarral, A.
dc.authorsophosFigueroa, A.
dc.authorsophosAnton-Aparicio, L. M.
dc.authorsophosCalvo, L.
dc.identifier.doi10.1186/1479-5876-10-186
dc.identifier.isi311108600001
dc.identifier.pmid22954417
dc.identifier.sophos7798
dc.journal.titleJournal of Translational Medicine
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario A Coruña::Anatomía Patolóxica
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario A Coruña::Oncoloxía médica
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña::INIBIC.- Instituto de Investigación Biomédica
dc.rights.accessRightsopenAccess
dc.rights.accessRightsopenAccess
dc.subject.decsBiomarcadores de Tumor
dc.subject.decsÁrea Bajo la Curva
dc.subject.decsMicroRNAs
dc.subject.decsReacción en Cadena de la Polimerasa
dc.subject.decsNeoplasias Gástricas 
dc.typesophosArtículo Original
dc.volume.number10


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