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dc.contributor.authorBarbazán, Jorge 
dc.contributor.authorBarbazán García, Jorge
dc.contributor.authorVieito Villar, María 
dc.contributor.authorAbalo Piñeiro, Alicia
dc.contributor.authorAlonso Alconada, Lorena
dc.contributor.authorMuinelo Romay , Laura
dc.contributor.authorAlonso Nocelo, Marta
dc.contributor.authorLeón Mateos, Luis Ángel 
dc.contributor.authorCandamio Folgar, Sonia 
dc.contributor.authorGallardo Martín, Maria Elena 
dc.contributor.authorAnido Herranz, Urbano 
dc.contributor.authorDoll, Andreas 
dc.contributor.authorde los Ángeles Casares, María
dc.contributor.authorGómez-Tato, Antonio
dc.contributor.authorAbal Posada, Miguel 
dc.contributor.authorLópez López, Rafael 
dc.date.accessioned2017-06-07T07:33:12Z
dc.date.available2017-06-07T07:33:12Z
dc.date.issued2012
dc.identifier.issn1582-1838
dc.identifier.urihttp://hdl.handle.net/20.500.11940/7856
dc.description.abstractThe accuracy in the diagnosis of metastatic colorectal cancer (mCRC) represents one of the challenges in the clinical management of patients. The detection of circulating tumour cells (CTC) is becoming a promising alternative to current detection techniques, as it focuses on one of the players of the metastatic disease and it should provide with more specific and sensitive detection rates. Here, we describe an improved method of detection of CTC from mCRC patients by combining immune-enrichment, optimal purification of RNA from very low cell numbers, and the selection of accurate PCR probes. As a result, we obtained a logistic model that combines GAPDH and VIL1 normalized to CD45 rendering powerful results in the detection of CTC from mCRC patients (AUROC value 0.8599). We further demonstrated the utility of this model at the clinical setting, as a reliable prognosis tool to determine progression-free survival in mCRC patients. Overall, we developed a strategy that ameliorates the specificity and sensitivity in the detection of CTC, resulting in a robust and promising logistic model for the clinical management of metastatic colorectal cancer patients.
dc.description.sponsorshipMinisterio de Sanidad, Consumo y Bienestar Social. Beca número: CP08/00142
dc.description.sponsorshipPrograma Comisión Europea Fondo Europeo de Desarollo Regional (FEDER)
dc.language.isoeng
dc.titleA logistic model for the detection of circulating tumour cells in human metastatic colorectal cancer
dc.typeArtigoes
dc.authorsophosBarbazan, J
dc.authorsophosVieito, M
dc.authorsophosAbalo, A
dc.authorsophosAlonso-Alconada, L
dc.authorsophosMuinelo-Romay, L
dc.authorsophosAlonso-Nocelo, M
dc.authorsophosLeon, L
dc.authorsophosCandamio, S
dc.authorsophosGallardo, E
dc.authorsophosAnido, U
dc.authorsophosDoll, A
dc.authorsophosde los Angeles Casares, M
dc.authorsophosGomez-Tato, A
dc.authorsophosAbal, M
dc.authorsophosLopez-Lopez, R
dc.identifier.doi10.1111/j.1582-4934.2012.01544.x
dc.identifier.isiWOS:000309237500010
dc.identifier.pmid22304365
dc.identifier.sophos7851
dc.issue.number10
dc.journal.titleJOURNAL OF CELLULAR AND MOLECULAR MEDICINE
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago::IDIS.- Instituto de investigaciones sanitarias de Santiago::Fundación Ramón Domínguez
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago - Complexo Hospitalario Universitario de Santiago::Oncoloxía médica
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago::IDIS.- Instituto de investigaciones sanitarias de Santiago
dc.page.initial2342
dc.page.final2349
dc.relation.projectIDMinisterio de Sanidad, Consumo y Bienestar Social/CP08/00142
dc.rights.accessRightsopenAccess
dc.typesophosArtículo Original
dc.volume.number16 (10)


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