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dc.contributor.authorRoca Rivada, Arturo
dc.contributor.authorBravo López, Susana Belén
dc.contributor.authorPérez Sotelo, Diego
dc.contributor.authorAlonso Lorenzo, Jana 
dc.contributor.authorCastro Pais, Ana Isabel
dc.contributor.authorBaamonde de la Torre, Ivan 
dc.contributor.authorBaltar Boileve, Javier 
dc.contributor.authorCasanueva Freijo, Felipe 
dc.contributor.authorPardo Pumar, María Isabel 
dc.date.accessioned2017-06-07T07:35:44Z
dc.date.available2017-06-07T07:35:44Z
dc.date.issued2015
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/20.500.11940/8341
dc.description.abstractIn the context of obesity, strong evidences support a distinctive pathological contribution of adipose tissue depending on its anatomical site of accumulation. Therefore, subcutaneous adipose tissue (SAT) has been lately considered metabolically benign compared to visceral fat (VAT), whose location is associated to the risk of developing cardiovascular disease, insulin resistance, and other associated comorbidities. Under the above situation, the chronic local inflammation that characterizes obese adipose tissue, has acquired a major role on the pathogenesis of obesity. In this work, we have analyzed for the first time human obese VAT and SAT secretomes using an improved quantitative proteomic approach for the study of tissue secretomes, Comparison of Isotope-Labeled Amino acid Incorporation Rates (CILAIR). The use of double isotope-labeling-CILAIR approach to analyze VAT and SAT secretomes allowed the identification of location-specific secreted proteins and its differential secretion. Additionally to the very high percentage of identified proteins previously implicated in obesity or in its comorbidities, this approach was revealed as a useful tool for the study of the obese adipose tissue microenvironment including extracellular matrix (ECM) remodeling and inflammatory status. The results herein presented reinforce the fact that VAT and SAT depots have distinct features and contribute differentially to metabolic disease.
dc.language.isoeng
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.meshAdipose Tissue
dc.subject.meshAmino Acids
dc.subject.meshExtracellular Matrix
dc.subject.meshHumans
dc.subject.meshInflammation
dc.subject.meshInflammation Mediators
dc.subject.meshInsulin Resistance
dc.subject.meshIntra-Abdominal Fat
dc.subject.meshIsotope Labeling
dc.subject.meshObesity
dc.subject.meshProteomics
dc.subject.meshSubcutaneous Fat
dc.titleCILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
dc.typeArtigoes
dc.authorsophosRoca-Rivada, A.
dc.authorsophosBravo, S. B.
dc.authorsophosPérez-Sotelo, D.
dc.authorsophosAlonso, J.
dc.authorsophosCastro, A. I.
dc.authorsophosBaamonde, I.
dc.authorsophosBaltar, J.
dc.authorsophosCasanueva, F. F.
dc.authorsophosPardo, M.
dc.identifier.doi10.1038/srep12214
dc.identifier.isi358304500002
dc.identifier.pmid26198096
dc.identifier.sophos19676
dc.issue.number12214
dc.journal.titleScientific Reports
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago - Complexo Hospitalario Universitario de Santiago::Cirurxía Xeral e dixestiva
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago - Complexo Hospitalario Universitario de Santiago::Endocrinoloxía
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de Santiago::IDIS.- Instituto de investigaciones sanitarias de Santiago
dc.rights.accessRightsopenAccess
dc.typesophosArtículo Original
dc.volume.number5


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Atribución 4.0 Internacional
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