DNA breakage detection-fluorescence in situ hybridization in buccal cells
Identificadores
Identificadores
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Visualización o descarga de ficheros
Fecha de publicación
2012Título de revista
EUROPEAN JOURNAL OF HISTOCHEMISTRY
Tipo de contenido
Artigo
DeCS
Mejilla | Daño del ADN | Relación Dosis-Respuesta a Droga | Técnicas Genéticas | Peróxido de Hidrógeno | Hibridación Fluorescente in Situ | AntioxidantesMeSH
DNA Damage | Dose-Response Relationship, Drug | Genetic Techniques | Hydrogen Peroxide | In Situ Hybridization, Fluorescence | Oxidants | CheekResumen
DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.