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dc.contributor.authorCortés-Gutiérrez, E.I 
dc.contributor.authorDávila-Rodríguez, M.I
dc.contributor.authorFernández García, José Luis 
dc.contributor.authorLópez-Fernández, C.
dc.contributor.authorGosálvez, J.
dc.date.accessioned2017-06-07T07:31:19Z
dc.date.available2017-06-07T07:31:19Z
dc.date.issued2012
dc.identifier.citationCortés-Gutiérrez EI, Dávila-Rodríguez MI, Fernández JL, López-Fernández C, Gosálvez J. DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells. Eur J Histochem. 2012 Dec 28;56(4):e49. doi: 10.4081/ejh.2012.e49. PMID: 23361245; PMCID: PMC3567768.
dc.identifier.issn1121-760X
dc.identifier.urihttp://hdl.handle.net/20.500.11940/7496
dc.description.abstractDNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.
dc.rightsAttribution-NonCommercial 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.meshDNA Damage 
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshGenetic Techniques 
dc.subject.meshHydrogen Peroxide 
dc.subject.meshIn Situ Hybridization, Fluorescence 
dc.subject.meshOxidants 
dc.subject.meshCheek 
dc.titleDNA breakage detection-fluorescence in situ hybridization in buccal cells
dc.typeArtigoes
dc.authorsophosCortés-Gutiérrez, E. I.
dc.authorsophosDávila-Rodríguez, M. I.
dc.authorsophosFernández, J. L.
dc.authorsophosLópez-Fernández, C.
dc.authorsophosGosálvez, J.
dc.identifier.doi10.4081/ejh.2012.e49
dc.identifier.isiWOS:000313464900011
dc.identifier.pmid233612
dc.identifier.sophos7497
dc.issue.number4
dc.journal.titleEUROPEAN JOURNAL OF HISTOCHEMISTRY
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña::INIBIC.- Instituto de Investigación Biomédica
dc.organizationServizo Galego de Saúde::Estrutura de Xestión Integrada (EOXI)::EOXI de A Coruña - Complexo Hospitalario Universitario A Coruña::Xenética
dc.rights.accessRightsopenAccess
dc.subject.decsMejilla 
dc.subject.decsDaño del ADN
dc.subject.decsRelación Dosis-Respuesta a Droga
dc.subject.decsTécnicas Genéticas
dc.subject.decsPeróxido de Hidrógeno
dc.subject.decsHibridación Fluorescente in Situ
dc.subject.decsAntioxidantes 
dc.typesophosArtículo Original
dc.volume.number56


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